Homeobox-containing transcription factor NANOG is essential in maintaining the pluripotent cells of the inner cell mass and in the derivation of embryonic stem cells (ESCs). Overexpression of NANOG is capable of maintaining the pluripotency and self-renewing characteristics of ESCs under what normally would be differentiation-inducing culture conditions. Concomitant with this essential function in pluripotent cell maintenance, expression of NANOG has restricted patterning in the inner cells of the morula, blastocyst, and epiblast during different developmental processes. Additionally, NANOG acts as a molecular gatekeeper to suppress or ensure differentiation in response to events in fluctuating environments and the presence of stimuli. NANOG has a single homeodomain that binds to DNA through the consensus sequence TAATGG. Coordinating with OCT4 and SOX2, NANOG regulates a large cohort of genes involved in pluripotency and development in later stages.

Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.