Gene Symbols:CCL20 | IFNG | IFNL2 | IL10 | IL12A | IL13 | IL17A | IL17F | IL1B | IL2| IL21 | IL22 | IL23A | IL4 | IL5 | IL6 | TGFB1 | TNF View more Hide
Number of Targets Detected:18
Compatible Sample Types:- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Method of Detection:Fluorescence Laser Scanner
Quantitative/Semi-Quantitative: Solid Support:Glass Slide
Size:1, 2, or 4 Slide Kit (16 sub-arrays per slide)
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
| Scroll over each target protein for more information |
|---|
IFN-gamma- Gene Symbol: IFNG
- Accession #: P01580
- ELISA Kit: RayBio Mouse IFN-gamma ELISA
| IL-1 beta(IL-1 F2)- Gene Symbol: IL1B
- Accession #: P10749
- ELISA Kit: RayBio Mouse IL-1 beta ELISA
| IL-10- Gene Symbol: IL10
- Accession #: Q0VBJ1
- ELISA Kit: RayBio Mouse IL-10 ELISA
| IL-12 p70- Gene Symbol: IL12A
- Accession #: P43431
| IL-13- Gene Symbol: IL13
- Accession #: P20109
- ELISA Kit: RayBio Mouse IL-13 ELISA
|
IL-17A- Gene Symbol: IL17A
- Accession #: Q60971
- ELISA Kit: RayBio Mouse IL-17A ELISA
| IL-17F- Gene Symbol: IL17F
- Accession #: Q7TNI7
- ELISA Kit: RayBio Mouse IL-17F ELISA
| IL-2- Gene Symbol: IL2
- Accession #: Q791T3
- ELISA Kit: RayBio Mouse IL-2 ELISA
| IL-21- Gene Symbol: IL21
- Accession #: E9PX58
- ELISA Kit: RayBio Mouse IL-21 ELISA
| IL-22- Gene Symbol: IL22
- Accession #: Q14BB3
- ELISA Kit: RayBio Mouse IL-22 ELISA
|
IL-23 p19- Gene Symbol: IL23A
- Accession #: Q9EQ14
- ELISA Kit: RayBio Mouse IL-23 alpha p19 ELISA
| IL-28A(IFN-lambda 2)- Gene Symbol: IFNL2
- Accession #: Q4VK74
- ELISA Kit: RayBio Mouse IL-28 ELISA
| IL-4- Gene Symbol: IL4
- Accession #: P07750
- ELISA Kit: RayBio Mouse IL-4 ELISA
| IL-5- Gene Symbol: IL5
- Accession #: P04401
- ELISA Kit: RayBio Mouse IL-5 ELISA
| IL-6- Gene Symbol: IL6
- Accession #: P08505
- ELISA Kit: RayBio Mouse IL-6 ELISA
|
MIP-3 alpha(CCL20)- Gene Symbol: CCL20
- Accession #: O89093
- ELISA Kit: RayBio Mouse MIP-3 alpha ELISA
| TGF beta 1- Gene Symbol: TGFB1
- Accession #: P04202
| TNF alpha- Gene Symbol: TNF
- Accession #: Q62326
- ELISA Kit: RayBio Mouse TNF-alpha ELISA
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Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Th1/Th2/Th17 Array Q2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
Learn MoreProtocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.