GATA transcription factors have a characteristic feature that they all bind to the DNA consensus sequence (A/T)GATA(A/G) of target gene promoters by two adjacent zinc fingers (Cys-X2-Cys-X17-Cys-X2-Cys). These transcription factors regulate differentiation, growth and survival of a wide range of cell types. The GATA family consists of six transcription factors, GATA1 to GATA6, between which the DNA-binding regions, two zinc finger domains are highly conserved. The C-terminal ‘‘C-finger’’ and its adjacent basic region are necessary and sufficient for GATA to bind to its cognate sequence. The N-terminal ‘‘N-finger’’ can also bind DNA independently, but has a preference for GATC core motifs. Both fingers participate in binding the palindromic GATA motif ATCWGATA (W = A/T), resulting in markedly increased affinity.

Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.