The RayBio® Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The RayBio® Custom Human Calreticulin-2 IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human Calreticulin-2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Calreticulin-2 coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and Calreticulin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. C_t values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower C_t values indicate a higher concentration of antigen.

May be stored for up to 6 months at 2^° to 8^°C from the date of shipment. Standard (recombinant protein) should be stored at -20^°C or -80^°C (recommended at -80^°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2^° to 8^°C. Note: the kit can be used within one year if the whole kit is stored at -20^°C. Avoid repeated freeze-thaw cycles.