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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
RayBiotech/Rapid Mouse Ig Isotyping Array 1/16 Sample Kit/AAM-ISO-G1-1
产品编号:AAM-ISO-G1-1
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 生化试剂>其他>>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: RayBiotech
公      司:RayBiotech
公司分类:
RayBiotech/Rapid Mouse Ig Isotyping Array 1/16 Sample Kit/AAM-ISO-G1-1
商品介绍

Antigen Information

Gene Symbols:
  • IGA
  • IGE
  • IGG2A
  • IGHG1
  • IGM

Assay Format

Number of Targets Detected:
10
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
16, 32, or 64 Sample Kit

Product Feature

  • One step mouse monoclonal antibody isotyping
  • Use only 1-2 µl sample
  • The whole experiment can be done within 1 hour
  • Sandwich based technology for high specificity and sensitivity
  • Low system CV with high reproducibility
  • High throughput sample processing
  • Qualitative visual inspection or semi-quantitative result
  • Processed slides can be stored for years without signal decay

Target Name

IgA, IgD, IgE, IgM, IgG1, IgG2a, IgG2b, and IgG3 Heavy Chains; Kappa and Lambda Light Chains

Target Species

Mouse

Application Notes

Suggested Application
Isotyping; Monoclonal Antibody Development; Hybridoma Screening and Selection; Biomarker Screening; Confirming Biological Processes
Kit Components
  • Mouse Ig Rapid Isotyping Glass Slide(s)
  • Sample Diluent
  • 20X Wash Buffer
  • Labeled Detection Antibody
  • Slide Washer/Dryer
  • 96-well Plate
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Mix samples with detection antibodies (in 96-well plate)
  3. Incubate with Sample/antibody mix (30 min at 37°C, followed by washes)
  4. Disassemble the glass slide
  5. Scan with a gene microarray laser scanner
  6. Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the Quantibody Array kit should be stored at -20 °C. At -20 °C the kit will retain complete activity for up to 6 months. The entire kit should be used within 6 months of purchase.
  1. Shao W., Zhang C., Liu E., et al. Identification of Liver Epithelial Cell-derived Ig Expression in ? chain-deficient mice. Sci Rep. 2016; 6: 23669. Published online 2016 Mar 29. doi: 10.1038/srep23669Species: MouseSample type: Tissue Lysate
  2. Groscurth S., Muller B., Schwan B., et al. Artificial forisomes are ideal models of forisome assembly and activity that allow the development of technical devices. Biomacromolecules. 2012 Oct 8;13(10):3076-86. doi: 10.1021/bm3008499Species: MouseSample type: Conditioned Media

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品牌介绍

RayBiotech

美国RayBiotech公司于2001年正式成立,由国际公认的蛋白芯片专家黄若磐教授一手创办,总部设在美国亚特兰大科技园。是世界上第一家专业从事蛋白芯片研发和生产的公司。


RayBiotech是一家领先的生命科学公司,提供蛋白质组学发现工具。RayBiotech拥有近150名员工,其目标是向RayBiotech客户(包括来自41个国家的工业界,学术界和研究机构的科学家)提供全球一流的产品和服务。RayBiotech致力于通过生命科学领域的创新和领导地位来加速客户成功。


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