RayBiotech/Human TGF beta Pathway Phosphorylation Array C1/2 Sample Kit/AAH-TGFB-1-2,RayBiotech,,RayBiotech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：RayBiotech/Human TGF beta Pathway Phosphorylation Array C1/2 Sample Kit/AAH-TGFB-1-2

产品编号：AAH-TGFB-1-2

市场价：¥0.00

场地：美国(厂家直采)

产品分类：生化试剂>缓冲液>其它缓冲液>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： RayBiotech

公司：RayBiotech

公司分类：

商品介绍

Antigen Information

Gene Symbols:

ATF2
FOS
JUN
MAP3K7
SMAD1
SMAD2
SMAD4
SMAD5

Assay Format

Pathway:

PKC Signaling
TGF-beta Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
c-Jun (P-Ser73) Gene Symbol: JUN Gene ID: 3725; Accession #: P05412	SMAD1 (P-Ser463/465) Gene Symbol: SMAD1 BSP1 MADH1 MADR1 Gene ID: 4086; Accession #: Q15797 ELISA Kit: RayBio Human Phospho-SMAD1 (SER463/465)	SMAD5 (P-Ser463/465) Gene Symbol: SMAD5 MADH5 Gene ID: 4090; Accession #: Q99717 ELISA Kit: RayBio Human Phospho-SMAD5 (SER463/465) ELISA	SMAD4 (P-Thr277) Gene Symbol: SMAD4 DPC4 MADH4 Gene ID: 4089; Accession #: Q13485 ELISA Kit: RayBio Human Phospho-SMAD4 (Thr277) ELISA	ATF2 (P-Thr69/71) Gene Symbol: ATF2 CREB2 CREBP1 Gene ID: 1386; Accession #: P15336 ELISA Kit: RayBio Custom Human Phospho-ATF2 (Thr69/71) and Total ATF2 Cell-Based ELISA
C-Fos (P-Thr232) Gene Symbol: FOS G0S7 Gene ID: 2353; Accession #: P01100 ELISA Kit: RayBio Human Phospho-C-Fos (Thr232) ELISA	SMAD2 (P-Ser245/250/255) Gene Symbol: SMAD2 MADH2 MADR2 Gene ID: 4087; Accession #: Q15796 ELISA Kit: RayBio Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) ELISA	TAK1 (P-Ser412) Gene Symbol: MAP3K7 TAK1 Gene ID: 6885; Accession #: O43318 ELISA Kit: Human Phospho-TAK1 (Ser412) ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human TGF beta Phosphorylation Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
500X HRP-Anti-Rabbit IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE"s ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Typical Data

Figure 1 - HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Calyculin A for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Poling, Holly M., et al. "Mechanically induced development and maturation of human intestinal organoids in vivo." Nature biomedical engineering 2.6 (2018): 429-442.Species: HumanSample type: Tissue Lysates

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Manual

SDS

Analysis Tool

品牌介绍

RayBiotech

美国RayBiotech公司于2001年正式成立,由国际公认的蛋白芯片专家黄若磐教授一手创办,总部设在美国亚特兰大科技园。是世界上第一家专业从事蛋白芯片研发和生产的公司。

RayBiotech是一家领先的生命科学公司，提供蛋白质组学发现工具。RayBiotech拥有近150名员工，其目标是向RayBiotech客户（包括来自41个国家的工业界，学术界和研究机构的科学家）提供全球一流的产品和服务。RayBiotech致力于通过生命科学领域的创新和领导地位来加速客户成功。

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