RayBiotech/Human/Mouse/Rat Phospho-CDK1 (Y15) Cell-Based ELISA/1 Plate Kit/CBEL-CDK1-Y15-1,RayBiotech,,RayBiotech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：RayBiotech/Human/Mouse/Rat Phospho-CDK1 (Y15) Cell-Based ELISA/1 Plate Kit/CBEL-CDK1-Y15-1

产品编号：CBEL-CDK1-Y15-1

市场价：¥0.00

场地：美国(厂家直采)

产品分类：其它>配件>温控控制>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： RayBiotech

公司：RayBiotech

公司分类：

商品介绍

Antigen Information

Accession Number:

P06493
P11440
P39951

Gene ID:

Gene Symbols:

CDC2
CDC28A
CDC2A
CDK1
CDKN1
P34CDC2

Protein Name & Synonyms:

Cyclin-dependent kinase 1(CDK1) Cell division control protein 2 homolog Cell division protein kinase 1 p34 protein kinase

Target Species:

Human
Mouse
Rat

Assay Format

Pathway:

p53 Signaling

Specificity:

The antibodies provided in this kit recognizes Human CDK1 phosphorylated at site Tyrosine-15.

Number of Targets Detected:

Species Detected:

Human
Mouse
Rat

Compatible Sample Types:

Adherent Cell Culture

Design Principle:

Cell-based

Method of Detection:

Colorimetric

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

96-well Microplate

Product Specifications

Size:

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Rapidly measure phosphorylated protein in adherent cell lines
Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
No sample lysis is needed
Compatible with a standard ELISA plate reader

Application Notes

Kit Components

Uncoated 96-well Strip Microplate
Wash Buffers
Fixing Solution
Quenching Buffer
Blocking Buffer
Anti-phospho antibody
Anti-pan antibody
HRP-Conjugated Secondary Antibody
TMB One-Step Substrate
Stop Solution

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacturer"s instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37^°C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

Upon receipt, the kit should be stored at â20^°C. Please use within 6 months from the date of shipment.

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Manual

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品牌介绍

RayBiotech

美国RayBiotech公司于2001年正式成立,由国际公认的蛋白芯片专家黄若磐教授一手创办,总部设在美国亚特兰大科技园。是世界上第一家专业从事蛋白芯片研发和生产的公司。

RayBiotech是一家领先的生命科学公司，提供蛋白质组学发现工具。RayBiotech拥有近150名员工，其目标是向RayBiotech客户（包括来自41个国家的工业界，学术界和研究机构的科学家）提供全球一流的产品和服务。RayBiotech致力于通过生命科学领域的创新和领导地位来加速客户成功。

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