Antigen Information
- P15336
- P16951
- 1386
- ATF2
- CREB2
- CREBP1
- Human
Assay Format
- MAPK Signaling
- PI3K-AKT Signaling
- TGF-beta Signaling
- Human
- Adherent Cell Culture
- Cell-based
- Semi-Quantitative
Product Specifications
Product Features
- Rapidly measure phosphorylated protein in adherent cell lines
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- No sample lysis is needed
- Compatible with a standard ELISA plate reader
Application Notes
- Uncoated 96-well Strip Microplate
- Wash Buffers
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Seed 10,000-30,000 cells into each well and incubate overnight.
- Apply various treatment, inhibitors or activators according to manufacturer"s instructions.
- Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
- Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
- Add 200 µl of Blocking Solution and incubate for 1 h at 37°C.
- Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
- Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
No posts found
Manual
SDS
RayBiotech
美国RayBiotech公司于2001年正式成立,由国际公认的蛋白芯片专家黄若磐教授一手创办,总部设在美国亚特兰大科技园。是世界上第一家专业从事蛋白芯片研发和生产的公司。
RayBiotech是一家领先的生命科学公司，提供蛋白质组学发现工具。RayBiotech拥有近150名员工，其目标是向RayBiotech客户（包括来自41个国家的工业界，学术界和研究机构的科学家）提供全球一流的产品和服务。RayBiotech致力于通过生命科学领域的创新和领导地位来加速客户成功。
产品列表：
没有。 | 品牌 | 货号 | 名称 | 规格 |
1 | 射线生物技术 | ELH-15PGDH-1 | 人15-PGDH / HPGD ELISA | 1套 |
2 | 射线生物技术 | PEL-4EBP1-T36-T-1 | 人/小鼠Phospho-4E-BP1（T36）和总4E-BP1 ELISA | 1套 |
3 | 射线生物技术 | ELH-41BBL-1 | 人4-1BB配体ELISA | 1套 |
4 | 射线生物技术 | ELH-2B4-1 | 人2B4 / CD244 ELISA | 1套 |
5 | 射线生物技术 | ELH-17bHSD1-1 | 定制人类17-beta-HSD1 / HSD17B1 ELISA | 1套 |
6 | 射线生物技术 | ELH-6Ckine-1 | 人6Ckine ELISA | 1套 |
7 | 射线生物技术 | ELH-A1BG-1 | 定制人类A1BG ELISA | 1套 |
8 | 射线生物技术 | QAH-ADI-1-1 | 人体肥胖阵列Q1 | 8个样品套件 |
9 | 射线生物技术 | QAH-ANG-1-1 | 人体血管生成阵列Q1 | 8个样品套件 |
10 | 射线生物技术 | QAH-ANG-2-1 | 人体血管生成阵列Q2 | 8个样品套件 |
11 | 射线生物技术 | 230-00001-10 | 重组人SAA | 10微克 |
12 | 射线生物技术 | 230-00002-50 | 重组人S100B | 50微克 |
13 | 射线生物技术 | 230-00003-白细胞 | 重组人类TIMP-1 | |
14 | 射线生物技术 | 230-00005-白细胞 | 重组人类TGF beta 1 | |
15 | 射线生物技术 | 230-00006-白细胞 | 重组人IL-10 |
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