RayBiotech/Human Adrenocorticotropic Hormone EIA/1 Plate Kit/EIA-ACTH-1,RayBiotech,,RayBiotech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：RayBiotech/Human Adrenocorticotropic Hormone EIA/1 Plate Kit/EIA-ACTH-1

产品编号：EIA-ACTH-1

市场价：¥0.00

场地：美国(厂家直采)

产品分类：仪器类>影像显示>细胞成像系统>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： RayBiotech

公司：RayBiotech

公司分类：

商品介绍

Antigen Information

Accession Number:

P01189

Gene ID:

5443

Gene Symbols:

POMC

Protein Name & Synonyms:

Pro-opiomelanocortin (POMC) (Corticotropin-lipotropin) [Cleaved into: NPP; Melanotropin gamma (Gamma-MSH); Potential peptide; Corticotropin (Adrenocorticotropic hormone) (ACTH); Melanotropin alpha (Alpha-MSH); Corticotropin-like intermediary peptide (CLIP); Lipotropin beta (Beta-LPH); Lipotropin gamma (Gamma-LPH); Melanotropin beta (Beta-MSH); Beta-endorphin; Met-enkephalin]

Assay Format

Detection Range:

0.1-1,000 ng/ml

Recommended Dilution (serum & plasma):

Human: 2X / Mouse: 2X / Rat: 2X

Sensitivity:

0.5 ng/ml

Specificity:

This EIA kit is designed to detect human, mouse, and rat ACTH

Number of Targets Detected:

Species Detected:

Human
Mouse
Rat

Compatible Sample Types:

Cell Culture Supernatants
Serum

Design Principle:

Competition-based

Method of Detection:

Colorimetric

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

96-well Microplate

Product Specifications

Size:

1, 2, or 5 x 96-Well Strip Microplate Kit

Introduction

Adrenocorticotropic hormone (ACTH, also adrenocorticotropin, corticotropin) is a 39 amino acids polypeptide tropic hormone. ACTH is synthesized from pre-pro-opiomelanocortin (pre-POMC), which is proteolytically cleaved by endopeptidases to yield various polypeptide fragments with varying physiological activity. ACTH is secreted by the anterior pituitary gland. It stimulates secretion of glucocorticoid steroid hormones from adrenal cortex cells, especially in the zona fasciculata of the adrenal glands. It influences steroid hormone secretion by both rapid short-term mechanisms and long-term actions. ACTH is also used as a medication and diagnostic agent.

Product Features

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Standard Peptide
Assay Diluent(s)
Biotinylated Peptide
HRP-Streptavidin
TMB One-Step Substrate
Stop Solution
Assay Diagram
Positive Control Sample
Capture Antibody
User Manual

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Orbital shaker
Aluminum foil
Saran Wrap
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Protocol Outline

Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at RT or O/N at 4°C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at RT.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at RT.
Add 100 µl TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.

Storage/Stability

Standard, Biotinylated ACTH peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

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Manual

SDS

品牌介绍

RayBiotech

美国RayBiotech公司于2001年正式成立,由国际公认的蛋白芯片专家黄若磐教授一手创办,总部设在美国亚特兰大科技园。是世界上第一家专业从事蛋白芯片研发和生产的公司。

RayBiotech是一家领先的生命科学公司，提供蛋白质组学发现工具。RayBiotech拥有近150名员工，其目标是向RayBiotech客户（包括来自41个国家的工业界，学术界和研究机构的科学家）提供全球一流的产品和服务。RayBiotech致力于通过生命科学领域的创新和领导地位来加速客户成功。

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