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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
RayBiotech/Rat Inflammation Array Q1/8 Sample Kit/QAR-INF-1-1
产品编号:QAR-INF-1-1
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 仪器类>洁净清洁>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: RayBiotech
公      司:RayBiotech
公司分类:
RayBiotech/Rat Inflammation Array Q1/8 Sample Kit/QAR-INF-1-1
商品介绍

Antigen Information

Gene Symbols:
  • CCL2
  • IFNG
  • IL10
  • IL13
  • IL1A
  • IL1B
  • IL2
  • IL4
  • IL6
  • TNF

Assay Format

Pathway:
  • JAK/STAT Signaling
  • MAPK Signaling
  • NFkB Signaling
Number of Targets Detected:
10
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
IFN-gamma
  • Gene Symbol: IFNG
  • Accession #: P01581
  • ELISA Kit: RayBio Rat IFN-gamma ELISA
IL-1 alpha(IL-1 F1)
  • Gene Symbol: IL1A
  • Accession #: P16598
  • ELISA Kit: RayBio Rat IL-1 alpha ELISA
IL-1 beta(IL-1 F2)
  • Gene Symbol: IL1B
  • Accession #: Q63264
  • ELISA Kit: RayBio Rat IL-1 beta ELISA
IL-2
  • Gene Symbol: IL2
  • Accession #: P17108
  • ELISA Kit: RayBio Rat IL-2 ELISA
IL-4
  • Gene Symbol: IL4
  • Accession #: P20096
  • ELISA Kit: RayBio Rat IL-4 ELISA
IL-6
  • Gene Symbol: IL6
  • Accession #: P20607
  • ELISA Kit: RayBio Rat IL-6 ELISA
IL-10
  • Gene Symbol: IL10
  • Accession #: P29456
  • ELISA Kit: RayBio Rat IL-10 ELISA
IL-13
  • Gene Symbol: IL13
  • Accession #: P42203
  • ELISA Kit: RayBio Rat IL-13 ELISA (For Lysates)
MCP-1(CCL2)
  • Gene Symbol: CCL2
  • Accession #: P14844
  • ELISA Kit: RayBio Rat MCP-1 ELISA
TNF alpha
  • Gene Symbol: TNF
  • Accession #: P16599
  • ELISA Kit: RayBio Rat TNF-alpha ELISA

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Rat Inflammation Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Bigelman E., Cohen L., Aharon-Hananel G., et al. Pathological presentation of cardiac mitochondria in a rat model for chronic kidney disease. PLoS One. 2018 Jun 11;13(6):e0198196. doi: 10.1371/journal.pone.0198196Species: RatSample type: Plasma
  2. Zheng S., Pan Y., Wang C., et al. HMGB1 Turns Renal Tubular Epithelial Cells into Inflammatory Promoters by Interacting with TLR4 During Sepsis. J Interferon Cytokine Res. 2016 Jan;36(1):9-19. doi: 10.1089/jir.2015.0067. Species: RatSample type: Conditioned Media
  3. Chang Y., Jia X., Sun X., et al. APRIL promotes proliferation, secretion and invasion of fibroblast-like synoviocyte from rats with adjuvant induced arthritis. Mol Immunol. 2015 Mar;64(1):90-8. doi: 10.1016/j.molimm.2014.11.002.Species: RatSample type: Conditioned Media
  4. Jiang S., Zhang M., Tian Z., et al. The monoacylglycerol lipase inhibitor JZL184 decreases inflammatory response in skeletal muscle contusion in rats. Eur J Pharmacol. 2015 Aug 15;761:1-10. doi: 10.1016/j.ejphar.2015.04.018.Species: RatSample type: Tissue Lysate
  5. Zhang M., Jiang S., Tian Z., et al. CB2R orchestrates fibrogenesis through regulation of inflammatory response during the repair of skeletal muscle contusion. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3491-502. eCollection 2015Species: RatSample type: Tissue Lysate
  6. Willett N., et al. Intra-articular injection of micronized dehydrated human amnion/chorion membrane attenuates osteoarthritis development. Arthritis Res Ther. 2014;16: R47.Species: RatSample type: Synovial Fluid
Great kit and easy to use
UNMC
Good
unmc
This array was very easy to use and provided great results for my project. The only downside was I did not have the appropriate scanner but the company offers complimentary scanning.
University of Florida
Write A Review

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#Analysis Tool

品牌介绍

RayBiotech

美国RayBiotech公司于2001年正式成立,由国际公认的蛋白芯片专家黄若磐教授一手创办,总部设在美国亚特兰大科技园。是世界上第一家专业从事蛋白芯片研发和生产的公司。


RayBiotech是一家领先的生命科学公司,提供蛋白质组学发现工具。RayBiotech拥有近150名员工,其目标是向RayBiotech客户(包括来自41个国家的工业界,学术界和研究机构的科学家)提供全球一流的产品和服务。RayBiotech致力于通过生命科学领域的创新和领导地位来加速客户成功。


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