RayBiotech/Porcine Cytokine Array Q1/8 Sample Kit/QAP-CYT-1-1,RayBiotech,,RayBiotech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：RayBiotech/Porcine Cytokine Array Q1/8 Sample Kit/QAP-CYT-1-1

产品编号：QAP-CYT-1-1

市场价：¥0.00

场地：美国(厂家直采)

产品分类：其它>耗材>盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： RayBiotech

公司：RayBiotech

公司分类：

商品介绍

Antigen Information

Gene Symbols:

CSF2
CXCL8
IFNG
IL10
IL12A
IL1B
IL4
IL6
TGFB1
TNF

Assay Format

Pathway:

JAK/STAT Signaling
MAPK Signaling
NFkB Signaling

Number of Targets Detected:

Species Detected:

Porcine (pig)

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF Gene Symbol: CSF2 Accession #: Q29118 ELISA Kit: RayBio Porcine GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P17803 ELISA Kit: RayBio Porcine IFN-gamma ELISA	IL-1 beta(IL-1 F2) Gene Symbol: IL1B Accession #: P26889 ELISA Kit: RayBio Porcine IL-1 beta ELISA	IL-10 Gene Symbol: IL10 Accession #: Q29055 ELISA Kit: RayBio Porcine IL-10 ELISA	IL-12 p70 Gene Symbol: IL12A Accession #: Q29053 ELISA Kit: RayBio Porcine IL-12 ELISA
IL-4 Gene Symbol: IL4 Accession #: Q04745 ELISA Kit: RayBio Porcine IL-4 ELISA	IL-6 Gene Symbol: IL6 Accession #: P26893 ELISA Kit: RayBio Porcine IL-6 ELISA	IL-8(CXCL8) Gene Symbol: IL8 Accession #: P26894 ELISA Kit: RayBio Porcine IL-8 ELISA	TGF beta 1 Gene Symbol: TGFB1 Accession #: P07200 ELISA Kit: RayBio Porcine TGF-beta-1 ELISA	TNF alpha Gene Symbol: TNF Accession #: P23563 ELISA Kit: RayBio Porcine TNF-alpha ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Porcine Cytokine Array Q1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Sohn EH., Jiao C., Kaalberg E., et al. Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study. Sci Rep. 2015 Jul 3;5:11791. doi: 10.1038/srep11791.Species: PorcineSample type: Vitreous Fluid
Goetz J., Fredericks D., Petersen E., et al. A clinically realistic large animal model of intra-articular fracture that progresses to post-traumatic osteoarthritis. Osteoarthritis Cartilage. 2015 Oct;23(10):1797-805. doi: 10.1016/j.joca.2015.05.022Species: PorcineSample type: Synovial Fluid
Beltran-Beck B., de la Fuente J., Garrido J., Aranaz A., Sevilla I., et al. Oral Vaccination with Heat Inactivated Mycobacterium bovis Activates the Complement System to Protect against Tuberculosis. PLOS One, 2014 May 19, DOI: 10.1371/journal.pone.0098048Species: PorcineSample type: Serum
Beltran-Beck B., Romero B., Boadella M., et al. Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis. Clin Vaccine Immunol. 2014 Aug;21(8):1128-36. doi: 10.1128/CVI.00221-14Species: PorcineSample type: Serum
Butz D., et al. The expired breath carbon delta value is a marker for the onset of sepsis in a swine model. Journal of Analytical Atomic Spectrometry. 2014, 29, 606-613. DOI: 10.1039/C3JA50340BSpecies: PorcineSample type: Plasma
Pazos, M. R., Mohammed, N., Lafuente, H., Santos, M., Martínez-Pinilla, E., Moreno, E., ... & Hillard, C. J. (2013). Mechanisms of cannabidiol neuroprotection in hypoxic?ischemic newborn pigs: Role of 5HT 1A and CB2 receptors.?Neuropharmacology,?71, 282-291.Species: PorcineSample type: Tissue Lysate
Mulloy DP, Stone ML, Crosby IK, et al.?Ex Vivo?Rehabilitation of Non-Heart-Beating Donor Lungs in a Preclinical Porcine Model: Delayed Perfusion Results in Superior Lung Function.?The Journal of thoracic and cardiovascular surgery. 2012;144(5):1208-1216. doi:10.1016/j.jtcvs.2012.07.056.Species: PorcineSample type: BAL Fluid

Sensitive and robust with good reproducibility. We took advantage of the free scanning services as well. Low price in comparison to others in the market.

Mallesh KurakulaUniversity of Memphis11/15/2019, 05:25 PM

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Manual

SDS

GAL

Analysis Tool

品牌介绍

RayBiotech

美国RayBiotech公司于2001年正式成立,由国际公认的蛋白芯片专家黄若磐教授一手创办,总部设在美国亚特兰大科技园。是世界上第一家专业从事蛋白芯片研发和生产的公司。

RayBiotech是一家领先的生命科学公司，提供蛋白质组学发现工具。RayBiotech拥有近150名员工，其目标是向RayBiotech客户（包括来自41个国家的工业界，学术界和研究机构的科学家）提供全球一流的产品和服务。RayBiotech致力于通过生命科学领域的创新和领导地位来加速客户成功。

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