RayBiotech/Mouse Cytokine Array Q1/8 Sample Kit/QAM-CYT-1-1,RayBiotech,,RayBiotech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：RayBiotech/Mouse Cytokine Array Q1/8 Sample Kit/QAM-CYT-1-1

产品编号：QAM-CYT-1-1

市场价：¥0.00

场地：美国(厂家直采)

产品分类：仪器类>破碎混匀>漩涡震荡仪>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： RayBiotech

公司：RayBiotech

公司分类：

商品介绍

Antigen Information

Gene Symbols:

CCL2 | CCL5 | CSF1 | CSF2 | CXCL1 | IFNG | IL10 | IL12A | IL13 | IL17A View more Hide

Assay Format

Pathway:

MAPK Signaling

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF Gene Symbol: CSF2 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IL-1 alpha(IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA	IL-1 beta(IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA
IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA	IL-17A Gene Symbol: IL17A Accession #: Q60971 ELISA Kit: RayBio Mouse IL-17A ELISA	IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA
IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-5 Gene Symbol: IL5 Accession #: P04401 ELISA Kit: RayBio Mouse IL-5 ELISA	IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA	IL-9 Gene Symbol: IL9 Accession #: P15247 ELISA Kit: RayBio Mouse IL-9 ELISA	KC(CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA
MCP-1(CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	M-CSF Gene Symbol: CSF1 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA	RANTES(CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	VEGF-A Gene Symbol: VEGFA Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF-A ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Mouse Cytokine Array Q1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Fink DM, Connor AL, Kelley PM, Steele MM, Hollingsworth MA, Tempero RM. Nerve Growth Factor Regulates Neurolymphatic Remodeling during Corneal Inflammation and Resolution. Ng LG, ed. PLoS ONE 2014;9(11):e112737. doi:10.1371/journal.pone.0112737.Species: MouseSample type: Tissue Lysate
Huang X, Ren L, Ye P, Cheng C, Wu J, et al. (2014) Peroxisome Proliferator Activated Receptor c Deficiency in T Cells Accelerates Chronic Rejection by Influencing the Differentiation of CD4+ T Cells and Alternatively Activated Macrophages. PLoS ONE 9(11): e112953. doi:10.1371/journal.pone.0112953Species: MouseSample type: Conditioned Media
Karra L., Haworth O., Priluck R., et al. Lipoxin B4 promotes the resolution of allergic inflammation in the upper and lower airways of mice. Mucosal Immunol. 2014 Dec 3. doi: 10.1038/mi.2014.116.Species: MouseSample type: Serum
Guo H., Yun C., Hou G., Du J., Huang X., et al. Mangiferin Attenuates Th1/Th2 Cytokine Imbalance in an Ovalbumin-Induced Asthmatic Mouse Model. PLOS One. Published: June 23, 2014. DOI: 10.1371/journal.pone.0100394Species: MouseSample type: Serum
Zingariello, Maria, et al. "Characterization of the TGF-?1 signaling abnormalities in the Gata1 low mouse model of myelofibrosis."?Blood?121.17 (2013): 3345-3363.Species: MouseSample type: Conditioned Media
Arevalo-Martin A., Molina-Holgado E., Guaza C. A CB?/CB? receptor agonist, WIN 55,212-2, exerts its therapeutic effect in a viral autoimmune model of multiple sclerosis by restoring self-tolerance to myelin. Neuropharmacology. 2012 Sep;63(3):385-93. doi: 10.1016/j.neuropharm.2012.04.012. Species: MouseSample type: Tissue Lysate
Robbins D., Li W., Humphrey K., Zhao Y. Redox Factor-1 Mediates Inflammatory Response during Tumor Promotion in Skin Epidermal JB6 Cells. Open Journal of Apoptosis, 2012, 1, 19-24. http://dx.doi.org/10.4236/ojapo.2012.13003Species: MouseSample type: Cell Lysate
Vila` L, Roglans N, Baena M, Barroso E, Alegret M, et al. (2012) Metabolic Alterations and Increased Liver mTOR Expression Precede the Development of Autoimmune Disease in a Murine Model of Lupus Erythematosus. PLoS ONE 7(12): e51118. doi:10.1371/journal.pone.0051118Species: MouseSample type: Conditioned Media
Kelley P., et al. Regressed lymphatic vessels develop during corneal repair. Lab Invest. 2011 Nov;91(11):1643-51. doi: 10.1038/labinvest.2011.121.Species: MouseSample type: Tissue Lysate
Ojeda, Gloria, et al. "Complement regulatory protein Crry/p65 costimulation expands natural Treg cells with enhanced suppressive properties in proteoglycan?induced arthritis."?Arthritis & Rheumatism?63.6 (2011): 1562-1572.Species: MouseSample type: Conditioned Media

Very easy! I recommend their scanning service!!!

Ondrej UherNIH08/30/2019, 04:07 PM

I used quite a a lot of cytokine and chemokine array kit from Raybiotech.These product is very simple and reliable to measure the production of cytokine/chemokine from cell culture media.They also provide a free Scanning service, so no need to worry about slide scanner. Highly recommended.

Sang LeeNational Institute of health09/04/2018, 01:28 PM

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品牌介绍

RayBiotech

美国RayBiotech公司于2001年正式成立,由国际公认的蛋白芯片专家黄若磐教授一手创办,总部设在美国亚特兰大科技园。是世界上第一家专业从事蛋白芯片研发和生产的公司。

RayBiotech是一家领先的生命科学公司，提供蛋白质组学发现工具。RayBiotech拥有近150名员工，其目标是向RayBiotech客户（包括来自41个国家的工业界，学术界和研究机构的科学家）提供全球一流的产品和服务。RayBiotech致力于通过生命科学领域的创新和领导地位来加速客户成功。

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